These images were obtained by Dr Valentina Caorsi using the FILM facility, and were published in the FILM calendar 2010.
They represent different views of skeletal muscle obtained with a Leica SP5 upright microscope. One image was obtained by Second Harmonic Generation and using two-photon excitation at 850 nm to alter the fibre shape. The other images were obtained by using fluorescent labels on actin, on the myosin essential light chain or on myosin's reactive thiol SH1. The 'square' was obtained by photobleaching, to investigate the existence of FRET between two of the labels, thus demonstrating that their labelling sites are less than 10 nm apart.
The Section of Molecular Medicine (MM) was created in August 2007, as a result of the merging of the sections of Molecular and Cellular Medicine and Biological Nanoscience. The Section is housed in the Sir Alexander Fleming Building on the South Kensington Campus of Imperial College. Most laboratories are found on the 2nd floor. MM regroups scientists with interests in fundamental aspects of cell and molecular biology, enhanced by expertise in physiology, biophysics, spectroscopy, single molecule imaging and state-of-the-art microscopies. The work extends research in the basic sciences at the National Heart and Lung Institute.
Current Principal Investigators in the Section include:
Research in the section is principally funded from programme and project grants from Research Councils (MRC, BBSRC) and charities (Wellcome Trust, British Heart Foundation and others).
Over sixty staff and students currently work in the Section, including nine heads of laboratories, over 20 PhD students, over 20 Postdocs and researchers, two technicians and a number of MRes students, visitors and honorary researchers. The Section Administrator is Catherine Enright (firstname.lastname@example.org; Desk 123, 2nd Floor, Sir Alexander Fleming Building)
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