Useful Links and Information
Flow cytometry links
Sites of general interest for flow cytometry
All links open in a new window
Core Facility websites within Imperial
Flow cyometry Facilities
- Department of Life Sciences, Flow Cytometry Facility South Kensington
Contact: Jane Srivastava
- MRC Hammersmith
Contact: Dr James Elliott
-
BRC Imaging and FACS Facility, Hammersmith
Contact: Dr Anna Ettorre
Imaging and other Core Facilities
- FILM
(Facility for Imaging by Light Microscopy) Contact: Martin Spitaler - for detailed information about imaging-related equipment see Imperial College Imaging Register - an invaluable inventory of microscopy tools and equipment in the College
- for other core facilities in Imperial College see Research Facilities Directory, main college website / Research or the Core Facility List of the Faculty of Medicine
- Electron Microscopy Centre
- Histology service (sample preparation, embedding, cutting, staining): contact Lorraine Lawrence
- Life Sciences Core Facilities
- Biological Imaging Centre, Hammersmith (non-invasive small animal live imaging with fluorescence / luminescence, MRI and PET)
User Groups
Formed in June 2006 to promote the interaction between all users of flow and imaging cytometry within the UK. They hold regional meetings to foster interaction between users and promote new applications and techniques. In addition this site acts as a central resource for links, protocols, meeting advertisments and other relevant information.
Huge, sprawling site with loads of information. Includes tutorials, reference materials, current protocols, and more. Home to the General Cytometry Email List (searchable emailing list with questions and answers), an international mailing list for flow cytometry users.
Regional cytometry users forum.
ISAC is the professional organization for scientists utilizing multidisciplinary, advanced technology for the measurement of cells and cell processes.
Basic Information
Free online text version of Howard Shapiro's textbook.
Excellent guide to compensation, how to do it, and why it's so important. Includes a quiz, many diagrams, and a short no-fail recipe for proper compensation every time.
A brief introduction to the history, science, and engineering behind flow cytometry from Science Park at the University of Texas MD Anderson Cancer Center.
Practical considerations
Biolegend provides an introduction to the various Brilliant Violet™ Antibodies and Brilliant Violet™ Considerations for Multi-color Flow Cytometry 
(downloadable PDF).
Brilliant Violet™ antibody conjugates, proudly co-developed by BioLegend and Sirigen, are an innovative class of novel research reagents, providing more options for your multicolor flow cytometry panels and getting you better results. Maximize the capacity of your violet laser with our large selection of directly labeled Brilliant Violet™ antibody conjugates.
- LEGENDScreen™ Human Cell Screening: fluorophore-conjugated antibodies provided in 96-well plates for the purpose of screening cell surface molecules on your cells of interest
Companies
- Flow Cytometry Technical Protocols: http://www.biolegend.com/support/prot
- Expression of Common Surface Molecules on Blood Cells
Tools
- Compensation - understanding how compensation works and why you need it.
- BDbiosciences spectrum viewer
- Invitrogen Spectrum Viewer
- FlowJo Tutorials
